Mission
The mission of the 组织学和影像学核心 is to provide COBRE investigators and the greater UNE research community access to expertise, training, 以及与组织处理相关的专业仪器, sectioning, staining, immunohistochemistry, and microscopy.
此外，Core还提供 services and training related to image analysis and image analysis software to guide investigators in choosing the best methods for presenting their data. 我们还为外部设施提供工作场所 服务收费的基础.
These images represent work completed by 组织学和影像学核心 professional staff. All samples were processed on core equipment, and images captured on core microscopes. 画廊包含各种各样的组织类型, 包括神经组织, bone, kelp, 还有果蝇幼虫.
Services
UNE COBRE组织学和影像学核心费用结构
| Service | Unit | Internal Fee | 外部学术费用 | 对外商业费用 |
|---|---|---|---|---|
| 杂项劳动、培训 | per hour | $37.50 | $58 | $116 |
| 脱钙，EDTA或甲酸 | per sample, week | $2.19 | $2.84 | $5.67 |
| 石蜡加工，标准 | per cassette | $1.52 | $1.95 | $3.91 |
| 石蜡加工，mega | per cassette | $2.03 | $2.46 | $4.91 |
| Embedding, Paraffin | per block | $1.97 | $2.93 | $5.86 |
| Embedding, Frozen | per block | $2.64 | $3.36 | $6.72 |
| 石蜡切片，常规 | per slide | $1.46 | $2.11 | $4.22 |
| 石蜡切片，切片 | per slide | $2.25 | $3.33 | $6.66 |
| 冷冻切片，每张片子最多3个切片 | per slide | $2.24 | $2.95 | $5.90 |
| 冷冻切片，每张幻灯片3或更多切片 | per slide | $3.55 | $4.99 | $9.98 |
| H&E staining, manual | per slide | $2.10 | $2.86 | $5.71 |
| H&E染色，自动染色 | per slide | $0.98 | $1.20 | $2.39 |
| 特殊染色，Tier 1 (TB, Picrosirius, PAS, Mucicarmine) | per slide | $2.74 | $3.95 | $7.90 |
| Special Staining, Tier 2 (Masson's Trichrome, Safranin O/Fast Green) | per slide | $4.13 | $5.88 | $11.76 |
| 免疫组化/免疫组化，石蜡(用户自备一抗) | per slide | $7.05 | $9.81 | $19.61 |
| IHC/I,F Frozen(用户自备一抗) | per slide | $5.65 | $7.86 | $15.73 |
Sample Processing
组织学和成像核心是完全集成与 UNE's Behavior Core and can directly process samples collected by the Behavior Core.
Training
The Core also provides training to professional staff and students on equipment and techniques.
The core has cryostats, microtomes, and microscopes available for reservation. 避免计划冲突和维护问题, 必须使用Outlook日历保留所有设备, 个人用户必须由核心工作人员进行培训.
The Leica confocal has a separate training procedure consisting of two 2-hour supervised sessions, 几个信息视频, and an online quiz. The first training session is an introduction to the microscope guided by core staff. The second session entails imaging of a user-generated sample with minimal intervention from Core staff. Upon completion of a proficiency quiz, users can reserve time as desired.
任何培训咨询，请发邮件给Peter Caradonna pcaradonna@szzhuodong.com.
Badges
The UNE Badging program is a non-credit bearing vehicle that digitally validates and documents knowledge, skill sets, qualifications, and competencies. Open to all learners, any participant who completes the learning activities and demonstrates achievement of the learning outcomes/competencies for a badge would be able to claim it. 与研究生院、潜在雇主等分享徽章., is a great way to demonstrate some of the skills and competencies you have acquired that are not captured in an academic transcript.
For interested students, the core is now part of the UNE Badging program. Students will gain a knowledge-base and skillsets relevant to the core specialty.
Funding
Assistance is available for help to cover the fees for work performed by the research cores.
Publications
Karamchedu NP, Fleming BC, Proffen BL, Sant NJ, Portilla G, Parola LR, Molino J, Murray MM. Terminal sterilization influences the efficacy of an extracellular matrix-blood composite for treating posttraumatic osteoarthritis in the rat model. J Orthop Res. 2022年3月,40 (3):573 - 583.
Eaton VE, Pettit S, Elkinson A, Houseknecht KL, King TE, May M. 小鼠卵巢切除术后多微生物性脓肿. BMC Vet Res. 2019 10月24日;15(1):364.
Sannajust S, Imbert I, Eaton V, Henderson T, Liaw L, May M, Barbe MF, King T. Females have greater susceptibility to develop ongoing pain and central sensitization in a rat model of temporomandibular joint pain. Pain. 2019年9月,160 (9):2036 - 2049.
葛尔斯维克K，福兰斯比T，甘特G. Bone Morphogenetic Protein Glass Bottom Boat (BMP5/6/7/8) and its receptor Wishful Thinking (BMPRII) are required for injury-induced allodynia in Drosophila. Molecular Pain. Jan-Dec 14:1744806918802703 2018;.
Sherman H, Gitschier HJ, Rossi AE. A Novel Three-Dimensional Immune Oncology Model for High-Throughput Testing of Tumoricidal Activity. Front. Immunol., 23 April 2018.
郝维林J，英伯特I，科米尔J，艾伦J，波雷卡F，金T. Central Sensitization and Neuropathic Features of Ongoing Pain in a Rat Model of Advanced Osteoarthritis. J Pain. 2016年3月,17 (3):374 - 82.
Deal A, Erickson K, Shiers S, Burman M, Limbic system development underlies the emergence of classical fear conditioning during the 3rd and 4th weeks of life in the rat. 行为神经科学. Behav Neurosci. 2016年4月,130 (2):212 - 30.
Warner E, Krivitsky R, Cone K, Atherton P, Pitre T, Lanpher J, Giuvelis G, Bergquist I, King T, Bilsky E, and Stevenson G. Evaluation of a Postoperative Pain-Like State n Motivated Behavior in Rats: Effects of Plantar Incision on Progressive-Ratio Food-Maintained Responding. Drug Dev Research. Dec 2015; 76(8):432–441.
Burman M, Bilsky E. FAAH inhibitor OL-135 disrupts contextual, but not auditory, fear conditioning in rats. Behav Brain Res. 2016 Jul 15;308:1-5.
孟门MA，西蒙斯CA，休斯M，雷L. Developing and validating trace fear conditioning protocols in C57BL/6 mice. J Neurosci Methods. 2014 Jan 30;222:111-7.
刘建军，曹磊. Morphine increases hippocampal viral load and suppresses frontal lobe CCL5 expression in the LP-BM5 AIDS model. J Neuroimmunol. 2014年4月15日;269(1-2):44-51.
Please remember to acknowledge the COBRE 组织学和影像学核心 and the financial support provided by the NIGMS grant number P20GM103643 in manuscripts, grants, 还有包括我们工作成果的演讲.
Contact
Location
Pickus 219/220
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教师和专业人员
